Natural Chemical Ind. Co., Ltd.

KINSASE and Animal Husbandry Production：

Due to its much higher growth rate than general agricultural investment, animal husbandry is the most important part of agriculture industry. Its rise or fall is decided by the amount of benefit reaped and that is related to the cost of the feed. The best way to reduce the cost is to supply sufficient and cheap feed. However, the feed demand in our country is increasing and most of it is imported. Hence, cheaper feed is hard to come by. Therefore, other methods must be found to reach the goal of reducing the cost. The most effective way is to use the same quantity and quality of feed to get the best result, i.e. with the most meat. The protein in most feeds, especially proteins feeds, cannot be totally absorbed and used by organisms and is wasted through the excrements. For this reason, our company, after many years of research, has successfully developed a compound protein decomposing enzyme to allow the protein in the feed to be broken down into short poly peptides, and then become part of the body. According to experiments, a unit weight of totally absorbed protein will produce five unit weight's worth of meat. This clearly shows that promoting absorption is more effective than increasing nutrients and, at the same time, makes better economic sense. Therefore, KINSASE can allow the farmers to endure less loss during lean times and get more profit when meat prices are high. It can further help feed makers to maintain the loans and raise the quality to increase its competitiveness.

Ingredients and functions of KINSASE：

Semi-Synthetic Protease：To break down the protein in the feed and raise the digestibility to improve feed efficiency. Each kilogram contains the transformation protein decomposing enzyme 150,000 units.
KINASE：A kind of enzyme for liver cell. It enhances detoxifying ability, speeds up the rate in which the RNA transports amino acid, raises protein synthesis rate, and increases muscle production.

Technical Breakthrough in KINSASE：

Using protein decomposing enzyme as the protein in the feed (excess protein in excrement from total formula feed) in an attempt to increase the effect of meat production and improve feed efficiency has been experimented by experts around the world for a long time. The first to be considered is pepsin. Through experiments, it has been proven to have significant effect on meat making. However, the high prices (tens of thousand dollars per kilogram) and low production amount (only 5-10g of pepsin can be obtained from the stomach of a fully grown cow.) make it impossible to be used in the feed. Then, enter the experiments using phyto-EMPV. Such substance has strong effect on protein decomposition, and it is fast working. The active functional groups in EMPV can break down poly peptides up to 12 times per second. Theoretically, it should have extremely high use value, but this substance has no obvious effect on weight gain for the same amount of feed in actual tests. One reason is that this kind of substance while breaking down the protein in the feed is being absorbed by the organism at the same time. After it is absorbed by the system, protein in the body is also quickly broken down. One cancels out the other and there is no significant improvement in meat production. Due to the drawbacks mentioned above, the molecular structure is altered to make it only work in intestines instead of in vivo cells. The modified protein decomposing enzyme will not be absorbed by the system, and thus autolysis is prevented. This significantly increases assimilation; therefore, this substance is a major breakthrough in feed efficiency and improving the weight gain rate. Its function may be expressed by the following diagram.

Comparison between protein decomposing enzyme：

Comparison between protein decomposing enzyme comparison after molecular change and the enzyme in original state：

Illustration：
The original protein decomposing enzyme can be quickly absorbed in intestines, be distributed through capillaries causing autolysis. Also because of fast absorption, the enzyme concentration in intestines rapidly reduces and decreases the effect on the protein. Therefore, muscle production effect is not obvious.

Illustration：
After the molecule has been changed, the protein decomposing enzyme will not be absorbed by the intestines, and therefore no autolysis. As the intestine wall does not absorb, there is a higher concentration of the enzyme in the intestine. It can be used to facilitate protein absorption in the feed and, through raising assimilation, making unprecedented progress in muscle production.

Mechanism of KINSASE：

Illustration：
From the above we know that reactants are the protein in the feed to products. Because the existence of decomposing enzyme, reaction occurs easily. Because the enzyme has catalytic effect, the activation is greatly reduced. Thus, the protein can be fully absorbed and used in the intestines. On the other hand, without enzyme's catalytic effect, it will need higher activation. It will work slowly and protein cannot be absorbed and used easily.

Illustration：
The above shows that when active-Groups in KINSASE (the added decomposing enzyme), such as -NH2, -SH, and -COO-, are mixed with Substrate (the protein in the feed), an effect similar to electromagnetic induction occurs causing the poly peptides in substrate to quickly collapse into a small unit protein or amino acid which can be absorbed easily. This work will be finished rapidly and continue to work in new substrate, keeping breaking down the protein. Therefore, a small amount of protein decomposing enzyme can allow a large amount of protein to be broken down and absorbed.

Experiments：

Eel

KINSASE in Eel Feed Experiment Result

Category	Processing situation	Pond number	Time	Specification	Breeding Number	Breeding weight	Captured weight	Weight increasing	Feed weight	Feed coefficient
Glass eel	KINSASE 0.3 %	N1	(4/20-6/17) 58days	507/Kg	14,000	27.6Kg	66.5Kg	38.8Kg	67.4Kg	1.73
Glass eel	KINSASE 0.3%	N2	(4/20-6/19) 60days	507/Kg	14,000	27.6Kg	55.6Kg	28Kg	71.7Kg	2.56
Glass eel	Control group	N3	(4/20-6/21) 62days	507/Kg	14,000	27.6Kg	41.14Kg	13.44Kg	54.13Kg	4.02
Eel	KINSASE 0.2%	S1	(6/4-8/16) 73days	84-97.5/Kg	24,315	277.8Kg	529.6Kg	251.7Kg	391.1Kg	1.55
Eel	Control group	S2	(5/9-8/23) 107days	24.5-32/Kg	16,754	640.1Kg	972.3Kg	332.1Kg	778.2Kg	2.34
Elver	KINSASE 0.2%	P1	(6/24-9/4) 72days	263-319/Kg	14,735	49.6Kg	109Kg	59.4Kg	138.6Kg	2.33
Elver	KINSASE 0.2%	P2	(6/24-9/8) 77days	776.4/Kg	18,746	24.1Kg	43.1Kg	19Kg	81Kg	4.26
Elver	Control group	P3	(6/24-9/10) 75days	680/Kg	17,864	26.2Kg	38.6Kg	12.4Kg	96.1Kg	7.7

Note：

Glass eel N1, N2,N3 were the stone eels bought from outside and immediately separated into three groups for experiment.
S1 and S2 were the eels that grown up in the normal environment.
P1,P2 and P3 were elvers with sluggish growth for unknown reasons.

Pig

Category	Number	Weight before experiment	Weight after experiment	Weight Increased	Weight increase per day	Average feedconsumed per pig	Feed efficiency
A ( + )	10	162.4kg	338.5kg	176.1kg	0.587kg	36.1	2.05
B ( + )	10	146.2kg	341.4kg	195.2kg	0.651kg	41.8	2.14
C ( - )	10	162 kg	322.4kg	160.4kg	0.535kg	37.2	2.32
D ( - )	10	170.4kg	334.8kg	164.4kg	0.548kg	37.5	2.28

One month: From Sep. 1 to Sep. 30
Raising management and disease control were carried in normal manners.
Experiment results and discussions:
The experiment groups increase on average 0.619 kg per day while the control groups increase 0.542 kg per day. From the above we know adding KINSASE can raise the weight increase rate up to 14.2%. The feed efficiency average in experiment groups is 2.1 and 2.3 in the control groups, increasing by 9.5%.It is not difficult to see that KINSASE does have substantive benefit on pig raising

Chickens

(1) Experimental branch: 10 days after the hybrid strain of chicks are hatched

Category	Number	Experiment period	Weight before experiment	Weight after experiment	Feed weight	Weight increasing	Feed efficiency	Days	Situation
Experiment group	1000	100days	86kg	2850 kg	7320 kg	2764 kg	7320/2764=2.64	110days	With 0.05% KINSASE
Control group	1000	100days	86kg	2370 kg	7115 kg	2284 kg	7115/2284=3.11	110days	Without any KINSASE

Review after adding KINSASE：

Weight increasing improvement rate (2764 - 2284) / 2284 X 100% = 20%
Feed efficiency improvement rate (3.11 - 2.64) / 2.64 X 100% = 17.8%
Profit and loss review
1. Quantity of KINSASE in this experiment 7320 X (0.5 / 100) = 3.66 (Kg)
2. Weight increase after adding 3.66kg of KINSASE 2764-2284=480 (Kg)

(2)The result after adding KINSASE in chicken feed

Category \ item	Number	Number of Death	Death rate (%)	Total weight increasd after 8 weeks(kg)	Feed weight (kg)	Feed Efficiency	Situation
The 1st Experiment group	1000	11	1	2278	5036	2.21	With 0.05 % of KINSASE
The 2nd Experiment group	1000	29	3	2195	4895	2.23	With 0.05 % of KINSASE
The 1st Control group	1000	52	5	1800	4770	2.65	Without any KINSASE
The 2nd Control group	1000	39	4	1864	4811	2.58	Without any KINSASE

Review of the experiment result：

The feed efficiency of each group is as above.
The average weight increasing improvement is 22%
The average feed efficiency improvement is 17.5%
The group with KINSASE added is 809kg heavier than others.
EA total of 4.96 kg of KINSASE was used in the experiment groups.

The effect after adding KINSASE in fresh water feed.

Experiment 1

Fish Experimented：German carp, take ground water fully oxygengated

Result

Category		Number	initial	2 weeks	4 weeks	6 weeks
Weight	Group A 0.1 % KINSASE	900	3450 / 3.83	5550 / 6.17	7575 / 8.41	8892 / 9.88	The average weight
Weight	Group B Without any KINSASE	900	2700 / 3.00	4300 / 4.78	5410 / 6.01	6350 / 7.06	Total
Growth coefficient	Group A	Growth coefficient =(Feed weight /Increasing weight)		2610/2100 =1.24	3043/2025 =1.50	3170/1417 =2.40	8823/5442 =1.62
Growth coefficient	Group B	Growth coefficient =(Feed weight /weight ga)		2368/1600 =1.48	2633/1600 =2.37	2800/940 =2.98	7801/3650 =2.14

German carps used in this experiment were bought from outside. Both its constitution and growth were not very good; therefore, the results of experiment were affected.

Experiment 2

Fish experimented：Red mouthbreeder, take the ground water, fully inflates.

Result

Category		Number	initial	10 days	20 days	30 days
Weight	Group A	500	6497 / 13.0	10290 / 21.0	16513 /33.7	21854 / 44.6	Average weight
Weight	Group B Without any KINSASE	500	6508 / 13.0	10114/ 20.6	15782 / 32.2	19288/ 40.2	Total
Growth coefficient	Group A 0.1 % KINSASE	Growth coefficient =(Feed weight /Increasing weight)		3421/3793 =0.90	5398/6223 =0.86	5293/5341 =0.99	14112/15357 =0.91
Growth coefficient	Group B	Growth coefficient =(Feed weight /weight ga)		5375/3796 =0.96	5498/5608 =0.98	5550/3506 =1.58	14623/12910 =1.13

Review of the experiments：

In the first experiment, the fry physiques are worse. Therefore, weight increase and growth coefficient were not satisfactory. However, under the same circumstances, the experimental groups have improved weight gain by up to 49% and the growth coefficient improved by 32%.
In the second experiment, for normal red mouthbreeders, weight gain improvement rate was 18.9% and the growth coefficient improved by 24%.

Dosage and Usage：

Pig 0.03-0.1%
Chicken 0.03-0.1%
Duck 0.03-0.05%
Goose 0.03-0.05%
Eel 0.1-0.2%
Shrimp 0.1-0.2%
Freshwater Water 0.05-0.1%

Method of use: Mix it with a little feed to double the amount, and then mixed well with all the feeds.

Features：

Raise feed efficiency by 10-15%.
Increase meat growth rate by 15-25%.
Promotes the shine and softness of hair and skin.
Reduce raising time.
Enhances the growth rate.
It can be used in long term generally with effect.
Enhance pig's lean meat rate.
Increase myoglobin making lean meat brighter and more tender.